draq7 dye Search Results


draq7 dna dye  (Carl Roth GmbH)
90
Carl Roth GmbH draq7 dna dye
CYTO-ID Red labeled human gingival fibroblasts were treated with or without chlorhexidine. CYTO-ID Red labeled gingival fibroblasts, fixed with 4% w/v PFA in PBS and subsequently stained with <t>DRAQ7</t> served as a positive control for the LIVE/DEAD staining: (A) CYTO-ID Red, (B) DRAQ7, and (C) their overlay. Labeled gingival fibroblasts without addition of chlorhexidine served as a negative control after DRAQ7 staining: (D) CYTO-ID Red, (E) DRAQ7, and (F) their overlay. CYTO-ID Red labeled gingival fibroblasts were treated with 132 mM chlorhexidine for 2 hours prior DRAQ7 staining: (G) CYTO-ID Red, (H) DRAQ7, and (I) their overlay. Arrows show live cells without DRAQ7 nucleus staining. The samples were examined under the CLSM (Leica TCS SP2). Scale bars: 100 μm.
Draq7 Dna Dye, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/draq7 dna dye/product/Carl Roth GmbH
Average 90 stars, based on 1 article reviews
draq7 dna dye - by Bioz Stars, 2026-05
90/100 stars
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CYTO-ID Red labeled human gingival fibroblasts were treated with or without chlorhexidine. CYTO-ID Red labeled gingival fibroblasts, fixed with 4% w/v PFA in PBS and subsequently stained with DRAQ7 served as a positive control for the LIVE/DEAD staining: (A) CYTO-ID Red, (B) DRAQ7, and (C) their overlay. Labeled gingival fibroblasts without addition of chlorhexidine served as a negative control after DRAQ7 staining: (D) CYTO-ID Red, (E) DRAQ7, and (F) their overlay. CYTO-ID Red labeled gingival fibroblasts were treated with 132 mM chlorhexidine for 2 hours prior DRAQ7 staining: (G) CYTO-ID Red, (H) DRAQ7, and (I) their overlay. Arrows show live cells without DRAQ7 nucleus staining. The samples were examined under the CLSM (Leica TCS SP2). Scale bars: 100 μm.

Journal: PLoS ONE

Article Title: Time resolved 3D live-cell imaging on implants

doi: 10.1371/journal.pone.0205411

Figure Lengend Snippet: CYTO-ID Red labeled human gingival fibroblasts were treated with or without chlorhexidine. CYTO-ID Red labeled gingival fibroblasts, fixed with 4% w/v PFA in PBS and subsequently stained with DRAQ7 served as a positive control for the LIVE/DEAD staining: (A) CYTO-ID Red, (B) DRAQ7, and (C) their overlay. Labeled gingival fibroblasts without addition of chlorhexidine served as a negative control after DRAQ7 staining: (D) CYTO-ID Red, (E) DRAQ7, and (F) their overlay. CYTO-ID Red labeled gingival fibroblasts were treated with 132 mM chlorhexidine for 2 hours prior DRAQ7 staining: (G) CYTO-ID Red, (H) DRAQ7, and (I) their overlay. Arrows show live cells without DRAQ7 nucleus staining. The samples were examined under the CLSM (Leica TCS SP2). Scale bars: 100 μm.

Article Snippet: The non-toxic DRAQ7 DNA dye was able to pass cell membrane of compromised cells, since they were fixed with 4% w/v PFA in PBS (335.2, Carl Roth GmbH, Germany) to generate a positive control of cell damage Consequently, the fibroblasts had damaged cell membranes and emitted both dyes, the CYTO-ID Red was located at the cell membranes and the DRAQ7 at the nuclei ( ).

Techniques: Labeling, Staining, Positive Control, Negative Control

CYTO-ID Red labeled human gingival fibroblasts were treated with different concentrations of chlorhexidine for 8 hours prior DRAQ7 staining. LIVE/DEAD stained gingival fibroblasts after treatment with 18 μM chlorhexidine: (A) CYTO-ID Red, (B) DRAQ7, and (C) their overlay. LIVE/DEAD stained gingival fibroblasts after treatment with 36 μM chlorhexidine: (D) CYTO-ID Red, (E) DRAQ7, and (F) their overlay. LIVE/DEAD stained gingival fibroblasts after treatment with 72 μM chlorhexidine: (G) CYTO-ID Red, (H) DRAQ7, and (I) their overlay. LIVE/DEAD stained gingival fibroblasts after treatment with 181 μM chlorhexidine: (J) CYTO-ID Red, (K) DRAQ7, and (L) their overlay. The samples were examined under the CLSM (Leica TCS SP2). Scale bars: 200 μm.

Journal: PLoS ONE

Article Title: Time resolved 3D live-cell imaging on implants

doi: 10.1371/journal.pone.0205411

Figure Lengend Snippet: CYTO-ID Red labeled human gingival fibroblasts were treated with different concentrations of chlorhexidine for 8 hours prior DRAQ7 staining. LIVE/DEAD stained gingival fibroblasts after treatment with 18 μM chlorhexidine: (A) CYTO-ID Red, (B) DRAQ7, and (C) their overlay. LIVE/DEAD stained gingival fibroblasts after treatment with 36 μM chlorhexidine: (D) CYTO-ID Red, (E) DRAQ7, and (F) their overlay. LIVE/DEAD stained gingival fibroblasts after treatment with 72 μM chlorhexidine: (G) CYTO-ID Red, (H) DRAQ7, and (I) their overlay. LIVE/DEAD stained gingival fibroblasts after treatment with 181 μM chlorhexidine: (J) CYTO-ID Red, (K) DRAQ7, and (L) their overlay. The samples were examined under the CLSM (Leica TCS SP2). Scale bars: 200 μm.

Article Snippet: The non-toxic DRAQ7 DNA dye was able to pass cell membrane of compromised cells, since they were fixed with 4% w/v PFA in PBS (335.2, Carl Roth GmbH, Germany) to generate a positive control of cell damage Consequently, the fibroblasts had damaged cell membranes and emitted both dyes, the CYTO-ID Red was located at the cell membranes and the DRAQ7 at the nuclei ( ).

Techniques: Labeling, Staining

(A) MIP of the live cell stain with CYTO-ID Red (green) and dead cell stain with DRAQ7 (red) at the different time points. Rectangles indicate area of zoomed in versions of each MIP. (B) Fluorescence intensity profile of the MIPs (see corresponding image above in A). The profile was measured top-down and averaged for the full width of the titanium implant. (C) The difference spectrum for consecutive profiles in B.

Journal: PLoS ONE

Article Title: Time resolved 3D live-cell imaging on implants

doi: 10.1371/journal.pone.0205411

Figure Lengend Snippet: (A) MIP of the live cell stain with CYTO-ID Red (green) and dead cell stain with DRAQ7 (red) at the different time points. Rectangles indicate area of zoomed in versions of each MIP. (B) Fluorescence intensity profile of the MIPs (see corresponding image above in A). The profile was measured top-down and averaged for the full width of the titanium implant. (C) The difference spectrum for consecutive profiles in B.

Article Snippet: The non-toxic DRAQ7 DNA dye was able to pass cell membrane of compromised cells, since they were fixed with 4% w/v PFA in PBS (335.2, Carl Roth GmbH, Germany) to generate a positive control of cell damage Consequently, the fibroblasts had damaged cell membranes and emitted both dyes, the CYTO-ID Red was located at the cell membranes and the DRAQ7 at the nuclei ( ).

Techniques: Staining, Fluorescence